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Immediately after forty eight hrs of transfection, cells ended up useful for qRT-PCR; western blotting verified knockdown. Each experiment was performed a minimum of in triplicate on three various instances.陶术要按顺序从左向右依次添加助溶剂。可配合物理方法,如涡流、超声波或热水浴使之帮助溶解。请测试